Detection Of Rankl Positive Cells in Gingival Tissue in Healthy & Chronic Periodontal Disease Patients
-A Comparative Study
Published: November 1, 2014 | DOI: https://doi.org/10.7860/JCDR/2014/.5125
J. Bhuvaneswarri, Bagavad Gita, S. C.Chandrasekaran
1. Reader, Department of Periodontics, Sree Balaji Dental College & Hospital, Chennai, India.
2. Professor, Department of Periodontics, Sree Balaji Dental College & Hospital, Chennai, India.
3. Professor & HOD, Department of Periodontics, Sree Balaji Dental College & Hospital, Chennai, India.
Correspondence
Dr. J. Bhuvaneswarri,
Reader, Department of Periodontics, Sree Balaji Dental College & Hospital,
Pallikaranai, Narayanapuram, Chennai-601 302, India.
Phone : 9994044047, E-mail : drbhuvana22@gmail.com
Aim & Objective: The receptor activator of NF-kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG), are the important proteins implicated in osteoclastogenesis. This study aimed to identify & quantify RANKL positive cells in gingival tissues in healthy & diseased patients & the study looks for immunohistochemical evidence of the expression of the protein in gingival tissue samples.
Materials and Methods: Patients were randomly selected. Thirty subject each for (test & control). Age range of 25-55y, either sex was selected. Tissue samples were collected from Control – Healthy Gingiva & Test-Chronic Periodontitis Patients. Tissue sections were prepared. An Immunohistochemical analysis was done & cell counting was done for RANKL positive cells.
Results: Immunohistochemical staining showed that RANKL-positive cells were significantly distributed in the inflammatory epithelium & connective tissue zone of diseased & non-diseased gingiva. RANKL, positive cells was increased significantly in patients with chronic periodontitis (p < 0.05).
Conclusion: These findings imply that in this comparative study of gingival tissue for, RANKL positive cells, these cells were present in both healthy & chronic periodontitis samples, but number of positive cells present is significantly increased in chronic periodontitis.
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